Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference.
Identifieur interne : 000496 ( Main/Exploration ); précédent : 000495; suivant : 000497Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference.
Auteurs : Nadia Bertazzon [Italie] ; Alessandro Raiola ; Carla Castiglioni ; Massimo Gardiman ; Elisa Angelini ; Michele Borgo ; Simone FerrariSource :
- Plant cell reports [ 1432-203X ] ; 2012.
Descripteurs français
- KwdFr :
- ARN double brin (MeSH), Acyltransferases (génétique), Acyltransferases (métabolisme), Agrobacterium tumefaciens (génétique), Extinction de l'expression des gènes (MeSH), Feuilles de plante (génétique), Feuilles de plante (microbiologie), Génétique inverse (MeSH), Interférence par ARN (MeSH), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Vitis (génétique), Vitis (microbiologie), Vitis (métabolisme).
- MESH :
- génétique : Acyltransferases, Agrobacterium tumefaciens, Feuilles de plante, Protéines végétales, Vitis.
- microbiologie : Feuilles de plante, Vitis.
- métabolisme : Acyltransferases, Protéines végétales, Vitis.
- ARN double brin, Extinction de l'expression des gènes, Génétique inverse, Interférence par ARN, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Acyltransferases (genetics), Acyltransferases (metabolism), Agrobacterium tumefaciens (genetics), Gene Expression Regulation, Plant (MeSH), Gene Silencing (MeSH), Plant Leaves (genetics), Plant Leaves (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), RNA Interference (MeSH), RNA, Double-Stranded (MeSH), Reverse Genetics (MeSH), Vitis (genetics), Vitis (metabolism), Vitis (microbiology).
- MESH :
- chemical , genetics : Acyltransferases, Plant Proteins.
- chemical , metabolism : Acyltransferases, Plant Proteins.
- genetics : Agrobacterium tumefaciens, Plant Leaves, Vitis.
- metabolism : Vitis.
- microbiology : Plant Leaves, Vitis.
- Gene Expression Regulation, Plant, Gene Silencing, RNA Interference, RNA, Double-Stranded, Reverse Genetics.
Abstract
Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene function by RNA interference (RNAi) in grapevine is difficult, because the generation of stable transgenic plants has low efficiency and is time consuming. Recently, transient expression of genes in grapevine leaves has been obtained by Agrobacterium tumefaciens infiltration (agroinfiltration). We therefore tested the possibility to silence grapevine genes by agroinfiltration of RNAi constructs. A construct to express a double strand RNA (dsRNA) corresponding to the defense-related gene VvPGIP1, encoding a polygalacturonase-inhibiting protein (PGIP), was obtained and transiently expressed by agroinfiltration in leaves of grapevine plants grown in vitro. Expression of VvPGIP1 and accumulation of PGIP activity were strongly induced by infiltration with control bacteria, but not with bacteria carrying the dsRNA construct, indicating that the gene was efficiently silenced. In contrast, expression of another defense-related gene, VST1, encoding a stilbene synthase, was unaffected by the dsRNA construct. We have therefore demonstrated the possibility of transient down-regulation of grapevine genes by agroinfiltration of constructs for the expression of dsRNA. This system can be employed to evaluate the effectiveness of constructs that can be subsequently used to generate stable RNAi transgenic plants.
DOI: 10.1007/s00299-011-1147-2
PubMed: 21932028
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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uniqKey="Castiglioni C" first="Carla" last="Castiglioni">Carla Castiglioni</name></author><author><name sortKey="Gardiman, Massimo" sort="Gardiman, Massimo" uniqKey="Gardiman M" first="Massimo" last="Gardiman">Massimo Gardiman</name></author><author><name sortKey="Angelini, Elisa" sort="Angelini, Elisa" uniqKey="Angelini E" first="Elisa" last="Angelini">Elisa Angelini</name></author><author><name sortKey="Borgo, Michele" sort="Borgo, Michele" uniqKey="Borgo M" first="Michele" last="Borgo">Michele Borgo</name></author><author><name sortKey="Ferrari, Simone" sort="Ferrari, Simone" uniqKey="Ferrari S" first="Simone" last="Ferrari">Simone Ferrari</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acyltransferases (genetics)</term><term>Acyltransferases 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(génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Vitis (génétique)</term><term>Vitis (microbiologie)</term><term>Vitis (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Acyltransferases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acyltransferases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Plant Leaves</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Acyltransferases</term><term>Agrobacterium tumefaciens</term><term>Feuilles de plante</term><term>Protéines végétales</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Leaves</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acyltransferases</term><term>Protéines végétales</term><term>Vitis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Gene Silencing</term><term>RNA Interference</term><term>RNA, Double-Stranded</term><term>Reverse Genetics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN double brin</term><term>Extinction de l'expression des gènes</term><term>Génétique inverse</term><term>Interférence par ARN</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene function by RNA interference (RNAi) in grapevine is difficult, because the generation of stable transgenic plants has low efficiency and is time consuming. Recently, transient expression of genes in grapevine leaves has been obtained by Agrobacterium tumefaciens infiltration (agroinfiltration). We therefore tested the possibility to silence grapevine genes by agroinfiltration of RNAi constructs. A construct to express a double strand RNA (dsRNA) corresponding to the defense-related gene VvPGIP1, encoding a polygalacturonase-inhibiting protein (PGIP), was obtained and transiently expressed by agroinfiltration in leaves of grapevine plants grown in vitro. Expression of VvPGIP1 and accumulation of PGIP activity were strongly induced by infiltration with control bacteria, but not with bacteria carrying the dsRNA construct, indicating that the gene was efficiently silenced. In contrast, expression of another defense-related gene, VST1, encoding a stilbene synthase, was unaffected by the dsRNA construct. We have therefore demonstrated the possibility of transient down-regulation of grapevine genes by agroinfiltration of constructs for the expression of dsRNA. This system can be employed to evaluate the effectiveness of constructs that can be subsequently used to generate stable RNAi transgenic plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21932028</PMID><DateCompleted><Year>2012</Year><Month>04</Month><Day>26</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>31</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference.</ArticleTitle><Pagination><MedlinePgn>133-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-011-1147-2</ELocationID><Abstract><AbstractText>Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene function by RNA interference (RNAi) in grapevine is difficult, because the generation of stable transgenic plants has low efficiency and is time consuming. Recently, transient expression of genes in grapevine leaves has been obtained by Agrobacterium tumefaciens infiltration (agroinfiltration). We therefore tested the possibility to silence grapevine genes by agroinfiltration of RNAi constructs. A construct to express a double strand RNA (dsRNA) corresponding to the defense-related gene VvPGIP1, encoding a polygalacturonase-inhibiting protein (PGIP), was obtained and transiently expressed by agroinfiltration in leaves of grapevine plants grown in vitro. Expression of VvPGIP1 and accumulation of PGIP activity were strongly induced by infiltration with control bacteria, but not with bacteria carrying the dsRNA construct, indicating that the gene was efficiently silenced. In contrast, expression of another defense-related gene, VST1, encoding a stilbene synthase, was unaffected by the dsRNA construct. We have therefore demonstrated the possibility of transient down-regulation of grapevine genes by agroinfiltration of constructs for the expression of dsRNA. This system can be employed to evaluate the effectiveness of constructs that can be subsequently used to generate stable RNAi transgenic plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bertazzon</LastName><ForeName>Nadia</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>CRA-VIT Centro di ricerca per la viticoltura, Conegliano, TV, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Raiola</LastName><ForeName>Alessandro</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Castiglioni</LastName><ForeName>Carla</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Gardiman</LastName><ForeName>Massimo</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Angelini</LastName><ForeName>Elisa</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Borgo</LastName><ForeName>Michele</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Ferrari</LastName><ForeName>Simone</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>233083</GrantID><Agency>European Research Council</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>09</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C081479">PGIP protein, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant 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Michele" sort="Borgo, Michele" uniqKey="Borgo M" first="Michele" last="Borgo">Michele Borgo</name><name sortKey="Castiglioni, Carla" sort="Castiglioni, Carla" uniqKey="Castiglioni C" first="Carla" last="Castiglioni">Carla Castiglioni</name><name sortKey="Ferrari, Simone" sort="Ferrari, Simone" uniqKey="Ferrari S" first="Simone" last="Ferrari">Simone Ferrari</name><name sortKey="Gardiman, Massimo" sort="Gardiman, Massimo" uniqKey="Gardiman M" first="Massimo" last="Gardiman">Massimo Gardiman</name><name sortKey="Raiola, Alessandro" sort="Raiola, Alessandro" uniqKey="Raiola A" first="Alessandro" last="Raiola">Alessandro Raiola</name></noCountry><country name="Italie"><noRegion><name sortKey="Bertazzon, Nadia" sort="Bertazzon, Nadia" uniqKey="Bertazzon N" first="Nadia" last="Bertazzon">Nadia Bertazzon</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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